RNA

Part:BBa_K5078002:Design

Designed by: Jonathan Kinross   Group: iGEM24_UVU-Utah-2   (2024-08-07)


PTC amiRNA


Assembly Compatibility:
  • 10
    INCOMPATIBLE WITH RFC[10]
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 12
    INCOMPATIBLE WITH RFC[12]
    Illegal NheI site found at 85
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    INCOMPATIBLE WITH RFC[23]
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 25
    INCOMPATIBLE WITH RFC[25]
    Illegal PstI site found at 112
    Illegal PstI site found at 205
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

We had to design the artificial micro RNA with the appropriate minimum length needed to order the construct from IDT and appropriate target sequences for the golden gate cloning process while not disrupting our target RNA sequence.


Source

We designed this part based off of the PTC1 mRNA sequence from Chlamydomonas reinhardtii.

References